Biotechnology And Molecular Biology

Skills acquired during my double masters in Biotechnology and Molecular Biology:

  • Biochemistry and Bio molecules: Qualitative analysis of carbohydrates – Glucose, fructose, galactose, maltose, lactose, sucrose. Qualitative analysis of amino acids – Tyrosine, tryptophan, phenylalanine, Methionine, serine, histidine, praline. Estimation of urea – DAM method, Estimation of protein by Lowry’s method, Separation of amino acids by paper chromatography, Thin layer chromatography
  • Microbiology:Media preparation, Inoculation Techniques, Subculturing, Identification and culturing of various organisms, Staining and enumeration of microorganisms, Assay of antibiotics production and demonstration of antibiotic resistance, Bacterial transformation and transduction, Diagnostic tests to identify various organisms, etc.
  • Genetic Engineering:Site directed mutagenesis, Primer designing and DNA sequencing
  • Molecular Biology:DNA isolation, RNA isolation, Western Blotting, different PCRs like RT-PCR, qPCR, Cloning, Sequencing, Mutagenesis, Plasmid isolation, SDS–PAGE, transformation, Two Dimensional gel electrophoresis. Restriction enzyme digestion Vector and Insert ligation, Transformation. Isolation, determination, purification and separation of protein, carbohydrates, and lipids
  • Immunology: ELISA, Real Time PCR (m-RNA expression)
  • Protein Biochemistry:Induction of NALP3 protein with IPTG and analysis on SDS – PAGE, Purification of protein on Ni-NTA column, Western blots
  • Plant Tissue Culture:Callus induction, Micro propagation, RNA and protoplast Isolation, Somatic embryogenesis, Regeneration of plantlets
  • Animal Cell Culture:Chick embryo fibroblast culture, Sub culturing of cells, Karyotyping

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